No functional contribution of the gustatory receptor, Gr64b, co-expressed in olfactory sensory neurons of Drosophila melanogaster

Chemosensation is essential for the survival of insects. Activities like searching food, mating, and oviposition in fruit fly, Drosophila melanogaster are to a great extent governed by chemical cues detected via olfaction gustation. This information conveyed higher brain centers populations diverse olfactory sensory neurons (OSNs) gustatory (GSNs) expressing receptors (ORs) (GRs), respectively. ORs exclusively expressed antenna maxillary palps, while GRs widely labellum, tarsi, genitalia etc. Interestingly, 14 were previously reported be D. . However, spatial expression pattern all their functional role still unclear. Recent data challenge dogma that single OSNs express OR. In present study, we studied 12 among structures on fly using Gal4-UAS binary system. We observed antennal nine out reported. Out these nine, consistent was only apparent Gr64b, reconfirmed its presence innervating three glomeruli lobe. These known innervated ab5A, ab5B ab8A OSNs, Next, generated double labeling crosses with Gr64b co-expression Or47a, which neuron. To elucidate co-expressed challenged Or47a-expressing wild type –/– mutant flies odor stimulation sensillum recording technique two satiation states (fed starved). Notably, did not observe any significant sensitivity or specificity changes mutants as compared flies. Taken together, our results reveal neurons, contribution GR this context remains obscure.